Coding

Part:BBa_K3001000:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-14)


Cas13a Lba Coding Sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 1609
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 1609
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1162
    Illegal BglII site found at 1223
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 1609
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1162
    Illegal PstI site found at 1609
    Illegal AgeI site found at 355
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protein is large and needs to be overexpressed using an E. coli that contains rare codons.


Source

Ordered from Addgene

References